NEST Biotechnology (formerly Wuxi NEST) offers a complete portfolio of sterile cell culture flasks, available in various sizes, surface treatments, and cap options—ideal for all your cell growth needs. Manufactured in ISO 100,000 cleanrooms using USP Class VI-grade virgin polystyrene, E-beam sterilized (SAL 10⁻⁶), DNase/RNase-free, and non-pyrogenic.
Key Features
Sizes & Growth Areas: 25, 75, 175, and 225 cm² for standard flasks; plus multi-layer (3‑layer and 5‑layer) formats for scale-up.
Surface Options: Tissue culture-treated (TC) for adherent cell lines, and non-treated for suspension cultures.
Cap Types:
Plug Seal: Screw-on, leak-resistant
Vent Cap: 0.22 µm hydrophobic membrane for gas exchange
Both cap types are available across sizes.
Design Features:
Frosted writing area with clear graduations
Notched bottoms for secure stacking
Packaged in sterile zip-bags
Traceable lot numbers for quality control
Certified under ISO 9001 & 13485
E-beam sterilization ensures ≥10⁶ sterility assurance
USP Class VI compliance, free of contaminants
Product Range & Model Codes
| Growth Area | Surface | Cap Type | Model No. | Qty/Pack | Qty/Case |
|---|---|---|---|---|---|
| 25 cm² | TC | Plug Seal | 707001 | 10 | 200 |
| 25 cm² | TC | Vent Cap | 707003 | 10 | 200 |
| 25 cm² | Non-TC | Plug Seal | 707011 | 10 | 200 |
| 25 cm² | Non-TC | Vent Cap | 707013 | 10 | 200 |
| 75 cm² | TC | Plug Seal | 708001 | 5 | 100 |
| 75 cm² | TC | Vent Cap | 708003 | 5 | 100 |
| 75 cm² | Non-TC | Plug Seal | 708011 | 5 | 100 |
| 75 cm² | Non-TC | Vent Cap | 708013 | 5 | 100 |
| 175 cm² | TC | Plug Seal | 709001 | 5 | 40 |
| 175 cm² | TC | Vent Cap | 709003 | 5 | 40 |
| 175 cm² | Non-TC | Plug Seal | 709011 | 5 | 40 |
| 175 cm² | Non-TC | Vent Cap | 709013 | 5 | 40 |
| 225 cm² | TC | Plug Seal | 721001 | 5 | 25 |
| 225 cm² | TC | Vent Cap | 721003 | 5 | 25 |
| 225 cm² | Non-TC | Plug Seal | 721011 | 5 | 25 |
| 225 cm² | Non-TC | Vent Cap | 721013 | 5 | 25 |
Multi-Layer Flasks (Tissue Culture Treated, Sterile):
3‑Layer (approx. 261 cm²): Model 731301 (Vent Cap) / 731302 (Plug Seal)
5‑Layer (approx. 435 cm²): Model 731001 (Plug Seal) / 731002 (Vent Cap), 100–150 mL medium, 8/case
Applications
Expansion of adherent/suspension cell cultures
Stem-cell and primary-cell research
Genetic engineering and transfection
Vaccine development and biopharma R&D
Higher throughput scale-up via multi-layer flasks
Please contact us should you need further information